Immobilization of Catalase onto Polylysine Modified Chitosan Polymer

In this study, chitosan polymer was modified in two different ways and used for the immobilization of bovine liver catalase (CAT). First, it activated with glutaraldehyde (GAL), then covalently immobilized onto support via lysine amino acid residue CAT. second modification, GAL-activated interacted polylysine (pLYS), reactivated GAL CAT immobilization. Samples bare (Chi), (ChiGAL), polylysine-modified (ChiGAL-pLYS), (ChiGAL-pLYSGAL) CAT-immobilized (ChiGAL -CAT ChiGAL-pLYSGAL-CAT) were all characterized by FTIR. The enzymatic activities Free CAT, ChiGAL ChiGAL-pLYSGAL-CAT samples investigated at pH temperatures values highest activity determined. addition, effect substrate concentration on under optimal conditions investigated. Optimum ChiGAL-CAT 7.5, 7.0 7.0, respectively; temperature determined as 25, 30 35 C, respectively. After immobilization, Vmax enzymes decreased, Km increased, efficiency to found be higher. It observed that after 20 repeatedly use column reactor, retained 88 % 76 their initial activities,